LOCUS       sequence-80416-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NRPS module containing CATE domains with N-terminal 6His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Brotherton CA, Balskus EP
  TITLE     A prodrug resistance mechanism is involved in colibactin 
            biosynthesis and cytotoxicity.
  JOURNAL   J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. 
            Epub 2013 Feb 20.
  PUBMED    23406518
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja312154m";
            journalName: "J Am Chem Soc"; date: "2013-03-6- 6"; volume: "135"; 
            issue: "9"; pages: "3359-62"
COMMENT     Sequence Label: pET28a-ClbN-NHis
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             96..128
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatgact ggtggacagc
      121 aaatgggtcg cggatccgaa ttcatgatgt cgggcaatcc gttgtcgtgg ccacaggaac
      181 agtgccacat cattgatcaa ctgtatcctt acagtgccgt aaacatcatt ggtggtgtgg
      241 tcaccattga gggaattgtc gatctgcccc gactacacgc ggccatccag tccgccattc
      301 ggcaatttga tgcgttgcgt atgtggtttg tgatgggaga ggagagtgaa gtggtcagcc
      361 aagtgcagcc ttatcactgg cgcgatatcc gccatttgac tttttcccca gattatgaca
      421 aggagaacct acgcccagcg gcgatcgaaa catttgttga tgagtggttc cggcagccct
      481 ttaccctgct ggcgcacgat ttgttcgagt ttgtcacctt tacgtgcggt gaacaataca
      541 gcggttattt atttaaagcc catcacggca ttgctgatgg ttggtcaatg gcgttgttgt
      601 caaaccatgt caagcgagca tacgaacagc aggacgtacc agatgatgcg tcaccagcct
      661 atagcgcatt tctggcgcag cagcaatctt atcaggcgtc aacgcgcttt gccgttgatc
      721 gtggctggtg gcgtgactat atcgacgaat accgcgactg ctttccagac agttccccca
      781 tcgttactac tgagggcatc agttgttcca cttggcttga gccagccatg attaaccgtc
      841 tctacaggtt gtgtaatcgt tatggctgta cgctta
//
