LOCUS       sequence-80417-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untagged, full-length protein with active site mutation Serine95 to 
            Alanine for in vivo peptidase assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Brotherton CA, Balskus EP
  TITLE     A prodrug resistance mechanism is involved in colibactin 
            biosynthesis and cytotoxicity.
  JOURNAL   J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. 
            Epub 2013 Feb 20.
  PUBMED    23406518
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja312154m";
            journalName: "J Am Chem Soc"; date: "2013-03-6- 6"; volume: "135"; 
            issue: "9"; pages: "3359-62"
COMMENT     Sequence Label: pET29b-ClbP-S95A
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga caataatgga acacgttagc
       61 attaaaacat tatatcatct cctgtgctgt atgctgctct ttatttccgc tatgtgcgct
      121 ttggcgcaag aacatgagcc tatcggggcg caagatgagc gcctgtcgac attaattcac
      181 caacggatgc aggaggccaa ggtcccagcc ctttccgtaa gtgtgaccat taagggggta
      241 cgtcagcgat ttgtctacgg tgttgccgat gtggctagtc agaaagcgaa tactctagac
      301 acagtttacg agctgggagc catgagtaag gcgtttaccg gacttgtggt gcaaatactg
      361 attcaggaag gcagactccg gcaaggggat gatatcatta cctatctgcc ggaaatgcgc
      421 ttgaattatc agggaaaacc tgcttccctg accgtggctg atttccttta tcatacatca
      481 ggattgcctt tttcaacact ggctcggctg gaaaacccta tgcctgggag cgctgtggca
      541 cagcaactgc gcaacgagaa tctgctgttt gcgccgggtg cgaagtttag ctatgcctcc
      601 gccaattatg atgtgttggg cgcggtgatt gaaaatgtga cgggaaaaac ctttacagag
      661 gtcattgcgg aacgactcac gcagccgctg ggcatgtcgg cgactgtggc agttaagggg
      721 gatgagatta ttgtcaacaa ggcaagcggc tataaactgg gattcggcaa acccgttctg
      781 tttcatgcgc ctctggcccg gaaccatgtt cctgccgcct atatccatag cactctgcct
      841 gatatggaaa tatggataga c
//
