LOCUS       sequence-80433-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DICE donor plasmid with the FOXA2 and OTX2 transcription factors. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attPC-FO
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggatgaacgg catgaacacg tacatgagca tgtcggcggc cgccatgggc agcggctcgg
       61 gcaacatgag cgcgggctcc atgaacatgt cgtcgtacgt gggcgctggc atgagcccgt
      121 ccctggcggg gatgtccccc ggcgcgggcg ccatggcggg catgggcggc tcggccgggg
      181 cggctggcgt ggcgggcatg gggccgcact tgagtcccag cctgagcccg ctcggggggc
      241 aggcggccgg ggccatgggc ggcctggccc cctacgccaa catgaactcc atgagcccca
      301 tgtacgggca ggcgggcctg agccgcgccc gcgaccccaa gacctacagg cgcagctaca
      361 cgcacgcaaa gccgccctac tcgtacatct cgctcatcac catggccatc cagcagagcc
      421 ccaacaagat gctgacgctg agcgagatct accagtggat catggacctc ttccccttct
      481 accggcagaa ccagcagcgc tggcagaact ccatccgcca ctcgctctcc ttcaacgact
      541 gtttcctgaa ggtgccccgc tcgcccgaca agcccggcaa gggctccttc tggaccctgc
      601 accctgactc gggcaacatg ttcgagaacg gctgctacct gcgccgccag aagcgcttca
      661 agtgcgagaa gcagctggcg ctgaaggagg ccgcaggcgc cgccggcagc ggcaagaagg
      721 cggccgccgg ggcccaggcc tcacaggctc aactcgggga ggccgccggg ccggcctccg
      781 agactccggc gggcaccgag tcgcctcact cgagcgcctc cccgtgccag gagcacaagc
      841 gagggggcct gggagagct
//
