LOCUS       sequence-80434-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniMos transposable element with PuroR, MCS cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1666
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     264..280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     264..280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    300..320
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    320..427
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     344..360
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     344..360
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(394..410)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..411)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(427..447)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(455..472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(455..471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attctatcag gtgtacaagt atgaaatgtc gtttcgaaca tatagatgtc tcgcaaacgt
       61 aaatatttat cgattgtcat aaaactttga ccttgtgaag tgtcaacctt gactgtcgaa
      121 ccaccatagt ttggcgcgaa ttgagcgtca taattgttta ctctcagtgc agtcaacatg
      181 tcgagtttcg tgccgaataa agagcaaacg cggacagtat taattttctg ttttcatttg
      241 aagaaaacag ctgcggccgg atccaggaaa cagctatgac catgattacg ccaagctatc
      301 aactttgtat agaaaagttg agctccaccg cggtggcggc cgctctagaa ctagtggatc
      361 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
      421 cggtaccaac tttattatac atagttgata attcactggc cgtcgtttta cagaggggag
      481 aaaatgcgga gagaagaacc cagcatctta ataaaattat ataattttga ataaaatcaa
      541 attaaaaata agattaattg aaacacaaca atagaatgca gaaaattact cataagaaat
      601 aatttatttt gaaagaagtt gtgtcgattt acgataaaaa ctacacgcaa acgaggctcc
      661 gcgtttacac cgtttggcaa tggagcgcgc ttgctattag attttaggcc agctcaacga
      721 aagtttaatt tatttttctt ctttttttcc atttttttga gtaaaatatc gaattttttg
      781 tattaattct gtaaatttta taatttgcaa ctattttggc gtattctgct tgtttttatc
      841 agcgaatttt tatgcattt
//
