LOCUS       sequence-80438-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniMos transposable element with HygroR, MCS cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1662
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     262..278
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     262..278
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    298..318
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    318..425
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     342..358
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     342..358
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(392..408)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(393..409)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(425..445)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(453..470)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(453..469)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tctatcaggt gtacaagtat gaaatgtcgt ttcgaacata tagatgtctc gcaaacgtaa
       61 atatttatcg attgtcataa aactttgacc ttgtgaagtg tcaaccttga ctgtcgaacc
      121 accatagttt ggcgcgaatt gagcgtcata attgtttact ctcagtgcag tcaacatgtc
      181 gagtttcgtg ccgaataaag agcaaacgcg gacagtatta attttctgtt ttcatttgaa
      241 gaaaacagct gcggccggat ccaggaaaca gctatgacca tgattacgcc aagctatcaa
      301 ctttgtatag aaaagttgag ctccaccgcg gtggcggccg ctctagaact agtggatccc
      361 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
      421 gtaccaactt tattatacat agttgataat tcactggccg tcgttttaca ggccggatat
      481 gaagatgaac gcaaggagcc tgatgcagcg actcttgcta ggagatcatc agaaagctta
      541 tatttgatta gtgacgtcgc atcaaagctg aacacataaa ttctttaata ttttatatcc
      601 ccatattatg cacctgagag gaatgaagaa atttgctgat actcccggtg aattcatgtc
      661 ttgatggttg tgcgcattgt ggtaatatct gaaaaatttc gaagtcagtt ttctgataag
      721 gcaacaacta ataaattgag aatcaattta cccttaagga acgatgttcg cttctgaatt
      781 tattaataaa tttttccgta attcaattca aaattgtcat atttaagaaa aaaacatcaa
      841 aaatgacaaa ttcaatattt taattaattt ttgctttcgt cgtaaatcta cacacgcgtc
      901 tcttccgtgc
//
