LOCUS       sequence-80439-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid with FEV (Ple67) promoter driving expression of iCre. 
            Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1991
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(7..29)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      156..611
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(243..262)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     453..474
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        637..741
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
       61 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      121 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
      181 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      241 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      301 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      361 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
      421 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
      481 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
      541 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
      601 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      661 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      721 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      781 tttgcggcat tttgccttcc tgtttttg
//
