LOCUS       sequence-80441-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E lysis protein that causes in subpopulation death when 
            threshold cell density is reached, resulting in oscillations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Marguet P, Tanouchi Y, Spitz E, Smith C, You L
  TITLE     Oscillations by minimal bacterial suicide circuits reveal hidden 
            facets of host-circuit physiology.
  JOURNAL   PLoS One. 2010 Jul 30;5(7):e11909. doi: 
            10.1371/journal.pone.0011909.
  PUBMED    20689598
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-07-30"; volume: "5(7):e11909. doi"; pages: " 
            10.1371/journal.pone.0011909"
COMMENT     Sequence Label: ePop
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(36..138)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    604..620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      121 cctcttacgt gccgatcaac gtctcatttt cgccagatat cgacgtcagt cctttgattc
      181 taataaattg gatttttgtc acactattgt atcgctggga atacaattac ttaacataag
      241 cacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaacgca
      301 agggaggttg gtatggtacg ctggactttg tgggataccc tcgctttcct gctcctgttg
      361 agtttattgc tgccgtcatt gcttattatg ttcatcccgt caacattcaa acggcctgtc
      421 tcatcatgga aggcgctgaa tttacggaaa acattattaa tggcgtcgag cgtccggtta
      481 aagccgctga attgttcgcg tttaccttgc gtgtacgcgc aggaaacact gacgttctta
      541 ctgacgcaga agaaaacgtg cgtcaaaaat tacgtgcgga aggagtgaga cgtctgtgtg
      601 gaattgtgag cggataacaa attcacacag ggccctcgga caccgaggag aatgtcaaga
      661 ggcgaacaca caacgtcttg gagcgccaga ggaggaacga gctaaaacgg agcttttttg
      721 ccctgcgtga ccagatcccg gagttggaaa acaatgaaaa ggcccccaag gtagttatcc
      781 ttaaaaaagc cacagcatac atcctgtccg tccaagcaga ggagcaaaag ctcatttctg
      841 aaga
//
