LOCUS       sequence-80456-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of human SirT1 in S. cerevisiae, HIS3 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ralser M, Zeidler U, Lehrach H
  TITLE     Interfering with glycolysis causes Sir2-dependent 
            hyper-recombination of Saccharomyces cerevisiae plasmids.
  JOURNAL   PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 
            2009 Apr 24.
  PUBMED    19390637
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(4):e5376. doi"; pages: " 
            10.1371/journal.pone.0005376. Epub 2009 Apr 24"
COMMENT     Sequence Label: p413TEF-SirT1
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..90
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     74..90
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttttcttgtt ctattacaac tttttttact tcttgctcat tagaaagaaa gcatagcaat
       61 ctaatctaag ttttctagaa ctagtggatc catggcggac gaggcggccc tcgcccttca
      121 gcccggcggc tccccctcgg cggcgggggc cgacagggag gccgcgtcgt cccccgccgg
      181 ggagccgctc cgcaagaggc cacggagaga tggtcccggc ctcgagcgga gcccgggcga
      241 gcccggtggg gcggccccag agcgtgaggt gccggcggcg gccaggggct gcccgggtgc
      301 ggcggcggcg gcgctgtggc gggaggcgga ggcagaggcg gcggcggcag gcggggagca
      361 agaggcccag gcgactgcgg cggctgggga aggagacaat gggccgggcc tgcagggccc
      421 atctcgggag ccaccgctgg ccgacaactt gtacgacgaa gacgacgacg acgagggcga
      481 ggaggaggaa gaggcggcgg cggcggcgat tgggtaccga gataaccttc tgttcggtga
      541 tgaaattatc actaatggtt ttcattcctg tgaaagtgat gaggaggata gagcctcaca
      601 tgcaagctct agtgactgga ctccaaggcc acggataggt ccatatactt ttgttcagca
      661 acatcttatg attggcacag atcctcgaac aattcttaaa gatttattgc cggaaacaat
      721 acctccacct gagttggatg atatgacact gtggcagatt gttattaata tcctttcaga
      781 accaccaaaa aggaaaaaaa gaaaagatat taatacaatt gaagatgctg tgaaattact
      841 gcaagagtgc aaaaaaatta tagttctaac tggagctggg gtgtctgttt catgtggaat
      901 acctgacttc aggtcaaggg atggtattta tgctcgtctt gctgtagact tcccagatct
      961 tccagatcct caagcgatgt ttgatattga atatttcaga aaagatccaa gaccattctt
     1021 caagtttgca aaggaaatat atcctggaca at
//
