LOCUS       sequence-80466-e        1539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry-based Calcium indicators.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Carlson HJ, Campbell RE
  TITLE     Circular permutated red fluorescent proteins and calcium ion 
            indicators based on mCherry.
  JOURNAL   Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 
            10.1093/protein/gzt052. Epub 2013 Oct 22.
  PUBMED    24151339
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/protein/gzt052"; journalName: "Protein Eng Des Sel"; date: 
            "2013-12"; volume: "26"; issue: "12"; pages: "763-72"
COMMENT     Sequence Label: CH-GECO2.1
FEATURES             Location/Qualifiers
     source          1..1539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..20
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             175..192
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..228
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(232..240,241..255)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             283..339
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     complement(776..794)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1044..1063
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggtttaa agaggagaaa ggtcatgaac aataacgatc tctttcaggc atcacgtcgg
       61 cgttttctgg cacaactcgg cagcttaacc gacgccggga cgctggggcc gtcattgtta
      121 acgccgcgac gtgcgactgc ggcgcaagcg gcgactgacg cttctagagg ttctcatcat
      181 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
      241 gacgatgatg ataaggatct cgccacaatg gtcgactcat cacgtcgtaa gtggaataag
      301 gcaggtcatg cagtcagagc tataggtcgg ctgagctcac ttgagtcgct gctgtccacc
      361 gagcggcttt accccgagga cggcgctctg aagggcgaga tcaagcagag gctgaagctg
      421 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
      481 cagctgcccg acgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac
      541 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      601 gagctgtaca agggtggcac tggcggttcc atggtgagca agggcgtgga ggataacatg
      661 gccttcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt caacggccac
      721 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      781 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      841 atgtacggct ccaaggccta cattaagcac ccagccgaca tccccgacta ctggaagctg
      901 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg aggtgtggtg
      961 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     1021 ggcaccaact tcccctccaa cggccccgta atgcagaaga agaccatggg ctggctgggc
     1081 ggtacgcgtg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
     1141 tttgacaagg gcggggctgg gacgataaca accaaggagc tggggacggt gatgcggtct
     1201 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1261 ggtgacggca cattagactt ccctgagttc ctgacgatga tggcaagaaa aatgaatgac
     1321 tcagacagtg aagaggaaat tagagaagcg ttccgtgtgt ttgataagga cggcaatggc
     1381 tacatcggcg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     1441 gaagaggttg atgaaatgat cagggtagca gacatcgatg gggatggtca ggtaaactac
     1501 gaagagtttg tacaaatgat gacagcgaag tgagaattc
//
