LOCUS       sequence-80470-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A improved cpmCherry variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Carlson HJ, Campbell RE
  TITLE     Circular permutated red fluorescent proteins and calcium ion 
            indicators based on mCherry.
  JOURNAL   Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 
            10.1093/protein/gzt052. Epub 2013 Oct 22.
  PUBMED    24151339
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/protein/gzt052"; journalName: "Protein Eng Des Sel"; date: 
            "2013-12"; volume: "26"; issue: "12"; pages: "763-72"
COMMENT     Sequence Label: cp196V1.2
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..71
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(75..83,84..98)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(412..430)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     680..699
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taaccatggg gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac
       61 agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg agcatgtcca
      121 gctccggggc actggtctgt tttatcaagt tggacatcac ctcacacaac gaggactaca
      181 ccatcgtgga acagtacgaa cgcgccgagg tccgccactc catcggcggc atggacgagc
      241 tgtacaaggg tggcactggc ggttccatgg tgagcaaggg cgtggaggat aacatggcct
      301 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtcaac ggccacgagt
      361 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      421 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagctcatgt
      481 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactactgg aagctgtcct
      541 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggaggc gtggtgaccg
      601 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      661 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
      721 ttgagcggtt gtaccccgag gacggcgctc tgaagggcga gatcaagcag aggctgaagc
      781 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcctg
      841 tgcagctgcc cggcgctcac aacgcgggcg gtgaccaact gacagaagag tgagaattc
//
