LOCUS       sequence-80475-e        4730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Nolte H, Holper S, Housley MP, Islam S, Piller T, Konzer A, Stainier
            DY, Braun T, Kruger M
  TITLE     Dynamics of zebrafish fin regeneration using a pulsed SILAC 
            approach.
  JOURNAL   Proteomics. 2015 Feb;15(4):739-51. doi: 10.1002/pmic.201400316.
  PUBMED    25504979
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pmic"; 
            journalName: "Proteomics"; date: "2015-02"; volume: "15"; issue: 
            "4"; pages: "739-51"
COMMENT     Sequence Label: pGEM-and1 in situ probe
FEATURES             Location/Qualifiers
     source          1..4730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1855..1873)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1856..1873)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1891..1907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1891..1907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1904..1926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1915..1931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1939..1945,1946..1963,1964..1969))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1984..2005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2122..2139)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2293..2881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2373..2392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3052..3843,3844..3912))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3675..3694
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3913..4017)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4095..4550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4232..4253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4444..4463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4676..4698
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4690..4707
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4691..4707
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4714..4730,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(4714..4730,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tatggctcat ttgagaggat attccatctt ccatgtgtta ctgcttgcaa cattaatatt
      121 accagcattt ctgctggctg gccccgtgac ccagcgttta aaaggagacg atggaagtga
      181 tgacaagaca ggccttgagg aagccccaaa gaaactgatc cgcaacagga gaaacatcag
      241 ttggtacaag cagcactctg acttctggaa ttggtacaaa tacttcactg acaatgagaa
      301 caaagacgga gttgcagagc tggatcgtgt ctatctgtca tacctgcaga acaagaatcg
      361 ggccgagtca cgtcgctcct acaagatgta cctgcagcac cttggtgaca tctacaagtc
      421 ctgtgctgag tctgatgacc caaactgtgt gtcctcatac accaacagac ccaagcccaa
      481 agctgaggcg ccagtgcctg ccccagtcaa gtcttgtgac ccttacagag acccatactg
      541 cttgtattca aagggatact catattaccc atacttggct ccagctcctg tcaaatctcc
      601 tgcacctgct cctgctccag ttaaagcccc ggcctacctt cacaccccag tggtcaagga
      661 cccacgttct ggtcaatact actactctcc attggtgcaa ccgttcctgt ccgctgaaca
      721 gcgggctgag ctgctgcgca tctgtgatgc agaggatgta gagtgtntcc agtaccacct
      781 ccgtgctgct tacggataca gatccgctgc tgctcttgcc ccgtcctatg cccatctcgg
      841 ttgtgaccct aagaccgatc ctcaatgtgt cccacatttg gtccagaaag ccccctctgg
      901 actttatctg cgttatccca actgtgaccc ccgagttgac ccatactgtg cttatgctgc
      961 tgctctggca tcctcgcaga accctgaatc tccaccctgc aaccctctct acgaagataa
     1021 ctgcaaccct ctgaccgcca caagaattgg cagtttggcc catgaaaatc tgaaggataa
     1081 gctcaaccct gaagctggcg ccatgcgggc acctcagatc cctcaacatg atccttatgc
     1141 catgttcagg gatgcatctg ctggggctga cctgcggaga cacatgcctg cacaactgca
     1201 acctccaagg taccaccagc cagaggagcc agagcaccct ttgggaccac gtgggaaaac
     1261 caaagaaggc tatgaatgtt tcgtcgggta tgataaagag tgcatcccac taagttccca
     1321 aaatgagcgc cgccctgctg tgcacagaca gccatcctac ccagccgaag cttatgagcc
     1381 acatctgaac gcagatggat ccaggactgg ggtgatcgag cctgatcccg actgtgaccc
     1441 agagtacgac agaaactgcc gcctgcgccg ctatgaacct gagcccgccg aacccgtcag
     1501 tatttcacct caagaggatg tggcccatcc agctcaggag ccatctcacc atgaggagat
     1561 ccctcagcat cgcgaggagt cctaccctga aacccctggc tatgaccagc agcaatatga
     1621 cgcctacatg agtggacaag aggatcccta tgccggctac ggcccccaag aaccaggagc
     1681 agccagtttt caggatgtcc tgagaggata tggtggtcgg tatcctggtc aggatgacca
     1741 ccttgcctac aatggagact acagaaagaa ataaaatcac tagtgaattc gcggccgcct
     1801 gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga gtattctata
     1861 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2701 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4021 atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4081 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4141 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4201 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4261 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4321 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4381 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4441 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4501 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     4561 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4621 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     4681 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
