LOCUS       sequence-80483-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of GFP (EF1a promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pEF-GFP
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          93..1035
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(116..136)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc
       61 ggtaccgtcg actgcagaat tcctcacgac acctgaaatg gaagaaaaaa actttgaacc
      121 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag
      181 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac
      241 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac
      301 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag
      361 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc
      421 gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca
      481 actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg
      541 ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc
      601 gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc
      661 gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct
      721 tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg
      781 aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca
      841 ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc
      901 tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggggcgt
      961 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc
     1021 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaa
//
