LOCUS       sequence-80486-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of DsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAG-DsRed
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(59..61,62..64,65..739)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(242..262)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     460..479
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     654..677
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(824..843)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    889..944
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(943..962)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ttggcaaaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
       61 ggtgcgctcc tccaagaacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
      121 caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      181 ccacaacacc gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
      241 cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc
      301 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      361 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggct gcttcatcta
      421 caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac
      481 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
      541 gatccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat
      601 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga
      661 catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgca ccgagggccg
      721 ccaccacctg ttcctgtagc ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg
      781 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
      841 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
      901 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
      961 ggagggcaaa tc
//
