LOCUS       sequence-80487-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of DsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAG-DsRed
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      170..179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(245..264)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(326..348)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gcgaagggca gggggccgcc cttggtcacc ttcagcttca cggtgttgtg gccctcgtag
       61 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacggt gccctccatg
      121 cgcaccttga agcgcatgaa ctccttgatg acgttcttgg aggagcgcac catggtggcg
      181 accggtggat cccgggcccg cggtaccgtc gactgcagaa ttctttgcca aaatgatgag
      241 acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag gcatgaacat
      301 ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc gccctgcccc
      361 gtcccccccg aaggcagccg tccccccgcg gacagccccg aggctggaga gggagaaggg
      421 gacggcggcg cggcgacgca cgaaggccct ccccgcccat ttccttcctg ccggcgccgc
      481 accgcttcgc cccgcgcccg ctagaggggg tgcggcggcg cctcccagat ttcggctccg
      541 cacagatttg ggacaaagga agtccctgcg ccctctcgca cgattaccat aaaaggcaat
      601 ggctgcggct cgccgcgcct cgacagccgc cggcgctccg ggggccgccg cgcccctccc
      661 ccgagccctc cccggcccga ggcggccccg
//
