LOCUS       sequence-80508-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of GFP-tagged LC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Fung C, Lock R, Gao S, Salas E, Debnath J
  TITLE     Induction of autophagy during extracellular matrix detachment 
            promotes cell survival.
  JOURNAL   Mol Biol Cell. 2008 Mar . 19(3):797-806.
  PUBMED    18094039
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2008 Mar . 19(3):797-806."
COMMENT     Sequence Label: pBABEpuro GFP-LC3
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..467
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFGTALAVTYMSALKATATGREPCL"
     primer_bind     complement(798..818)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc tagaccgtcc gagaagacct
       61 tcaaacagcg ccggagcttc gaacaaagag tggaagatgt ccggctcatc cgggagcagc
      121 accccaccaa gatcccagtg attatagagc gatacaaggg tgagaagcag ctgcccgtcc
      181 tggacaagac caagttcctt gtacctgatc acgtgaatat gagcgaactc atcaagataa
      241 ttagaaggcg cctgcagctc aatgctaacc aagccttctt cctcctggtg aatgggcaca
      301 gcatggtgag tgtgtccaca cccatctctg aagtgtacga gagcgagaga gatgaagacg
      361 gcttcctgta catggtctat gcctcccagg agacgttcgg gacagcactg gctgttacat
      421 acatgtcagc tctgaaggca acagcaacag gaagagagcc atgcttgtga cagacataca
      481 gccacttcca actaaagcaa gcctctgctc cctgctacct gcatggagcc cactgtgaca
      541 ctcagaccat ccccggtcac tcactcactc gtgtctgaga atctcagtga gagctgcctc
      601 tgtcacggac ggaagccaac acagccacct ctcgacctgg ctccccacag cacccaccct
      661 ccctgcatgc agctgtccct gctaaccccc aatgttatgt tacactgtgt aagtccccac
      721 tgctgccgtg tgtgggttgt gtacgtcgtc atgtccctgg tttataacta tggtgcgaat
      781 tctgcagtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggcagaag
      841 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      961 aactccgccc atcccgcccc taac
//
