LOCUS       sequence-80509-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  p2attNG landing pad plasmid with 400 bp homology arms to the human 
            H11 locus (for use with TALEN-assisted recombination)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attNG-H11-short
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..193
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     primer_bind     complement(726..745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(727..745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(752..769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(752..768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(761..783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttccgaagtt cctattcttc aaaaggtata ggaacttcaa gcttctaacc ccgcatacca
       61 agaaaccccc tacccggccc gcgaaggcta ggtagggggc tttttgtgtt tcagtgggta
      121 tggccgtgat gacctgtgtc ttcgtggttt gtctggtcaa ccaccgcggt ctcagtggtg
      181 tacggtacaa acccatgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      241 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggcggcc
      301 aattccatag ttgatttctc ctaaatcaag atagagtcca attaactttt tttttttttt
      361 aagatggagt ctcactctgt cgccaggctg gactgcagtg gcgtgatctc agctcactgc
      421 aacctctgcc tcccaggttc aggcgattct tctgtctcag cctcccgagt agctgggact
      481 acaagtgtgg gccaccatgc ccagctagtt ttgtattttt agtagagatg ggatttcacc
      541 atcttggcta ggatggtctt gatctcttga ccttgtgatt cgcctgcctc tgcctcccag
      601 agtgctgaga ttacaggcgt gagccacagt gcccaatttc catgggtttt caagaaaaac
      661 ttaacttact taaacttcag atcacttcct taaaggaaca tactggcgcg cctgtaccca
      721 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      781 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acat
//
