LOCUS       sequence-80511-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses U6 RNA, using its own pol III enhancer sequence and tagged
            with the self splicing intron neo cassette .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
  TITLE     The RNA polymerase dictates ORF1 requirement and timing of LINE and 
            SINE retrotransposition.
  JOURNAL   PLoS Genet. 2009 Apr;5(4):e1000458. doi: 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
  PUBMED    19390602
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2009-04"; volume: "5(4):e1000458. doi"; pages: " 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24"
COMMENT     Sequence Label: pU6-neo-TET
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(96..115)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 agtgaggagg cttttttgga ggtcgagcca atatgggatc ggccattgaa caagatggat
       61 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      121 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      181 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact cgagtactcc aaacctaatc
      241 aatatacttt cgcatacaaa ttaggtccca gcggctccag tgttgcatca cttcatccgc
      301 tagctcccat taaggagagg tccgactata tcttatgaga agaatacatc ttccccgacc
      361 gacatttagt ctgtgaactg catccatatc aacagaagat ctgttgactt aggacttggc
      421 tgcgtggtta ggaccatgtc cgtcagctta ttaccatacc ctttgcaagg ccatctcaaa
      481 gtttcccctg agacttggta ctgaacggct gttgacccct ttcccgcaat ttgacggtct
      541 tgccttttaa accgatgcaa tctattggtt taaagactag ctaccaggtg catgcctgat
      601 aacttttccc tgcaattcat tatattgcta attacaggac gaggcagcgc ggctatcgtg
      661 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
      721 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
      781 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatcc
//
