LOCUS       sequence-80515-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of tdTomato controlled by the ubiquitously 
            active CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Waldner C, Roose M, Ryffel GU
  TITLE     Red fluorescent Xenopus laevis: a new tool for grafting analysis.
  JOURNAL   BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.1186/1471-213X-9-37.
  PUBMED    19549299
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2009-06-23"; volume: "9:37. doi"; pages: " 
            10.1186/1471-213X-9-37"
COMMENT     Sequence Label: pCSCMV:tdTomato
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..20
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     28..44
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..44
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     63..83
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        65..83
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    196..330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(233..252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     287..306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     363..379
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     364..380
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(487..510)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(685..704)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     954..972
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
       61 gcgcaattaa ccctcactaa agggaacaaa agctgggtac cgggcccaat gcattggcgc
      121 cgcggccgcg aattaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      181 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      241 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      301 ctcatcaatg tatcttatca tgtctggatc tacgtaatac gactcactat agttctagag
      361 gctcgaggtc gacggtatcg ataagcttga tatcgaattc ttacttgtac agctcgtcca
      421 tgccgtacag gaacaggtgg tggcggccct cggagcgctc gtactgttcc acgatggtgt
      481 agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag ccgggcagtt
      541 gcacgggctt cttggccatg tagatggtct tgaactccac caggtagcgg ccgccgtcct
      601 tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct
      661 cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg ggggggaagt
      721 tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag gagtcctggg
      781 tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
      841 ggaaggacag cttcttgtaa tcggggatgt cggcggggtg cttcacgtac gccttggagc
      901 cgtacatgaa ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
      961 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc c
//
