LOCUS       sequence-80519-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, 
            Tabar V, Mo Q, Studer L, Sadelain M
  TITLE     Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and 
            c-Myc expression for efficient human iPSC induction and 
            differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
  PUBMED    19549847
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Aug 4. 106(31):12759-64."
COMMENT     Sequence Label: pLM-vexGFP-Oct4
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..41
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        23..41
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        69..295
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             296..476
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    523..648
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
       61 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
      121 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      181 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      241 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      301 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      361 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      421 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      481 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      541 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      601 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      661 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      721 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      781 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      841 acaggatcag aagaactta
//
