LOCUS       sequence-80528-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable suppression of miR-31 function.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC,
            Brock JE, Richardson AL, Weinberg RA
  TITLE     A pleiotropically acting microRNA, miR-31, inhibits breast cancer 
            metastasis.
  JOURNAL   Cell. 2009 Jun 12. 137(6):1032-46.
  PUBMED    19524507
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 12. 137(6):1032-46."
COMMENT     Sequence Label: pBABE-puro-mIR-31 sponge
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(25..27,28..30,31..741,742..747,748..870)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(70..91)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(331..350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
       61 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      181 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      241 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      481 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      601 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      721 ggcatggacg agctgtacaa gaagcttagc catggcttcc cgccggaggt ggaggagcag
      781 gatgatggca cgctgcccat gtcttgtgcc caggagagcg ggatggaccg tcaccctgca
      841 gcctgtgctt ctgctaggat caatgtgtag gcggccgcct cgagagctat gcctcgtctt
      901 gcctccggag ctatgcctcg
//
