LOCUS       sequence-8057-ex       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3632..4276
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4170..4193
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     4282..4298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4282..4298
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4306..4430
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4455..4460,4461..4478,4479..4485)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4539..5198
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4605..4624)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5543..5848
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5727..5746
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5889..6013)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(6045..6047,6048..6050,6051..6761)
                     /codon_start=1
                     /product="monomeric optimized variant of ECFP (Kremers et 
                     al., 2006)"
                     /label=SCFP3A
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6090..6111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6351..6370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6698..6719
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      6771..7018
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6787..6805)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7036..7055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7037..7055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7065..7082)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7065..7081)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7074..7096)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7226..7681
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7313..7332)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7523..7544
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7981..8385
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8398..9492
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9985..10004)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10104..10126
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cttcgagttt atcattatca atactcgcca tttcaaagaa
     3661 tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa attagccttt
     3721 taattctgct gtaacccgta catgcccaaa atagggggcg ggttacacag aatatataac
     3781 accgaaggtg cctgggtgaa caggtttatt cctggcatcc actaaatata atggagcccg
     3841 ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca
     3901 ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac
     3961 aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac
     4021 acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct
     4081 gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt
     4141 cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct
     4201 atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac
     4261 cagaacttag tttcgacgga ttctagaact agtggatccc ccatcacaag tttgtacaaa
     4321 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     4381 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     4441 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     4501 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     4561 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     4621 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     4681 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     4741 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     4801 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     4861 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     4921 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     4981 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     5041 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     5101 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     5161 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gccgcgtgga tccggcttac
     5221 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     5281 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     5341 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     5401 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     5461 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     5521 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     5581 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     5641 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     5701 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     5761 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     5821 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     5881 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     5941 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     6001 tgtacaaagt ggtgatgggc tgcaggaatt cgatatcaag cttaatggtg agcaagggcg
     6061 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     6121 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     6181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     6241 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     6301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     6361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     6421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     6481 acatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     6541 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     6601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     6661 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     6721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaactcgag tcatgtaatt
     6781 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     6841 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     6901 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
     6961 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg
     7021 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     7081 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     7141 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     7201 cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7261 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7321 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7381 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7441 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7501 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7561 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7621 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     7681 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatcgacgg
     7741 tcgaggagaa cttctagtat atccacatac ctaatattat tgccttatta aaaatggaat
     7801 cccaacaatt acatcaaaat ccacattctc ttcaaaatca attgtcctgt acttccttgt
     7861 tcatgtgtgt tcaaaaacgt tatatttata ggataattat actctatttc tcaacaagta
     7921 attggttgtt tggccgagcg gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt
     7981 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta
     8041 tttttttcct caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat
     8101 gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa
     8161 aaattgggag aaaaaggaaa ggtgagaggc cggaaccggc ttttcatata gaatagagaa
     8221 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
     8281 tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt
     8341 acatttcagc aatatatata tatatttcaa ggatatacca ttctaatgtc tgcccctatg
     8401 tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc
     8461 gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc
     8521 gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgtcccact tccagatgag
     8581 gcgctggaag cctccaagaa ggttgatgcc gttttgttag gtgctgtggc tggtcctaaa
     8641 tggggtaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa
     8701 ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca
     8761 atcaagccac aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt
     8821 atttactttg gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa
     8881 tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat
     8941 gagccaccat tgcctatttg gtccttggat aaagctaatc ttttggcctc ttcaagatta
     9001 tggagaaaaa ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat
     9061 caattgattg attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt
     9121 ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt
     9181 tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt
     9241 ggtttgtacg aaccatgcca cggttctgct ccagatttgc caaagaataa ggttgaccct
     9301 atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa
     9361 ggtaaggcca ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat
     9421 ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa
     9481 atccttgctt aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa
     9541 attcataata gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac
     9601 tatatacgca atctacatac atttatcaag aaggagaaaa aggaggatag taaaggaata
     9661 caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt gcactttaac
     9721 attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag aaaatcatga
     9781 aactacgatt cctaatttga tattggagga ttttctctaa aaaaaaaaaa atacaacaaa
     9841 taaaaaacac tcaatgacct gaccatttga tggagtttaa gtcaatacct tcttgaagca
     9901 tttcccataa tggtgaaagt tccctcaaga attttactct gtcagaaacg gccttacgac
     9961 gtagtcgata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
    10021 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    10081 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    10141 accgaaacgc gcga
//
