LOCUS       sequence-80570-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Zeocin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-zeo (pBABE-bleo)
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(58..78)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        63..392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      243..378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             402..776
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ggccggatcc cagtgtggtg gtacgtagga attcgccagc acagtggtcg accctgtgga
       61 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      121 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      181 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      241 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      301 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
      361 gaggcttttt tggaggccta ggcttttgca aaaagcttac catggccaag ttgaccagtg
      421 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
      481 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
      541 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
      601 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
      661 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
      721 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgaccga
      781 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
      841 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
      901 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
      961 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     1021 gctgaatatg ggc
//
