LOCUS       sequence-80582-e        3135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Candida codon-optimized dTomato with NAT 
            selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Gratacap RL, Rawls JF, Wheeler RT
  TITLE     Mucosal candidiasis elicits NF-kappaB activation, proinflammatory 
            gene expression and localized neutrophilia in zebrafish.
  JOURNAL   Dis Model Mech. 2013 Sep;6(5):1260-70. doi: 10.1242/dmm.012039. Epub
            2013 May 29.
  PUBMED    23720235
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dmm.012039"; journalName: "Dis Model Mech"; date: 
            "2013-09"; volume: "6"; issue: "5"; pages: "1260-70"
COMMENT     Sequence Label: pENO1-dTom-NATr
FEATURES             Location/Qualifiers
     source          1..3135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      1725..1912
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1780..1804)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1984..2327
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2906..3103
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 acttgtgggt ttaacatcat ttgtatcttt agtagacatg attgtgcaat accgtgatta
       61 tttgttttga aaggtctgtc atatttctat caatttcaaa acaaaatgtt catcagaaaa
      121 aagccaaaaa tgtctcttct agtttcttag tggtgtcgca taatacacaa tgtcgctcaa
      181 caatccacat tcccggcgca tagctcaaat cacatgacta cagctaacaa ttacacaaaa
      241 aaaattctct ttttgatgta gcaactatct tcaactaaaa cattttctcc ttcggcccat
      301 gattgtcctc cgggtcgaca gcaagccgtt acaattgaga tggaaagcga cctaccttca
      361 ctcgataagg tgcttaattg tacttcatat aaatctggcc cggatctaaa caaatgagtt
      421 ccattaagcc gtgggttctc aattagggtt tttgtttttg atttagaaaa aagagatcaa
      481 gatttgttta caggtgatgc ctttttttag aacttatgcg ttgcaaaagt tgactaacga
      541 tttctataag gtgatccaca ctaattatac aaacgtacaa gcggccgcac ttttcctgcg
      601 ttcacctgat gttggccaga tttctctctt cattgcatag aacataacca cactagggca
      661 acagaaaaaa aaaaaaaaag tgcatcggga agttgtgttc cattcattat atgtctacta
      721 ctgcatataa gtagcccacc caccaccacc atagtaagtt tttgtgtatg cgcgccgtca
      781 ggttatttca tttctgaatt tttcaaccac cttactccct ttattgttga ttgacaattt
      841 tgctcacagt aagttctttt agactccaat taatataaaa taagtctgat tttccaattc
      901 ctgttttttc tttttttttc tgtttctatt tctttccttt tctccctttt ttttaattct
      961 tcattcaatc atcaattgat aattcaggaa tattacaacc atggtttcaa aaggtgaaga
     1021 agttattaaa gaatttatga gatttaaagt tagaatggaa ggttcaatga atggtcatga
     1081 atttgaaatt gaaggtgaag gtgaaggtag accttatgaa ggtactcaaa ctgctaaatt
     1141 aaaagttact aaaggtggtc cattaccttt tgcttgggat attttatcac cacaatttat
     1201 gtatggttca aaagcatatg ttaaacatcc agctgatatt ccagattata aaaaattatc
     1261 atttccagaa ggttttaaat gggaaagagt tatgaatttt gaagatggtg gtttagttac
     1321 tgttactcaa gattcatcat tacaagatgg tactttaatt tataaagtta aaatgagagg
     1381 tactaatttt ccaccagatg gtccagttat gcaaaaaaaa actatgggtt gggaagcatc
     1441 aactgaaaga ttatatccaa gagatggtgt tttaaaaggt gaaattcatc aagcattgaa
     1501 attgaaagat ggtggtcatt atttggttga atttaaaact atttatatgg ctaaaaaacc
     1561 agttcaattg ccaggttatt attatgttga tactaaattg gatattactt cacataatga
     1621 agattatact attgttgaac aatatgaaag atctgaaggt agacatcatt tgtttttgta
     1681 tggtatggat gaattgtata aataattaat taacttctaa ataagcgaat ttcttatgat
     1741 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
     1801 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
     1861 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
     1921 ctagggataa cagggtaata tgatatcatg cttgcctcgt ccccgccggg tcacccggcc
     1981 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     2041 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     2101 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct acagacctgc
     2161 gagcagggaa acgctcccct cacagacacg ttgaattgtc cccacgccgc gcccctgtag
     2221 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     2281 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg tctactactt
     2341 tggatgatac tgcttataga tacagaactt ctgttccagg tgatgctgaa gctattgaag
     2401 cattggatgg ttctttcact accgatactg tttttagagt tactgctact ggtgatggtt
     2461 tcactttgag agaagttcca gttgatccac cattgactaa ggttttccca gatgatgaat
     2521 ccgatgatga atccgatgct ggtgaagatg gtgatccaga ttcaagaact ttcgttgctt
     2581 atggtgatga tggtgatttg gctggtttcg ttgttgtttc ttattctggt tggaacagaa
     2641 gattgactgt tgaagatatt gaagttgctc cagaacatag aggtcatggt gttggtagag
     2701 ctttgatggg tttggctact gaatttgcca gagaaagagg tgctggtcat ttgtggttgg
     2761 aagttaccaa tgttaatgct ccagctattc atgcttatag aagaatgggt ttcactttgt
     2821 gtggtttgga tactgcttta tacgatggta ctgcttccga tggtgaacaa gcattgtata
     2881 tgtctatgcc atgtccataa tcagtactga caataaaaag attcttgttt tcaagaactt
     2941 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     3001 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     3061 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     3121 acgccgccat ccagt
//
