LOCUS       sequence-80588-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Hygromycin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-hygro
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..461)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(61..77)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(93..115)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    662..861
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ctgtgctggc gaattcctac gtaccaccac actgggatcc ggccggcgcc tagagaagga
       61 gtgagggctg gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga
      121 gacggggcgg atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag
      181 ggaggggggt caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt
      241 ccatgcgggc caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc tcggttaaag
      301 gtgccgtctc gcggccatcc gacgttaaag gttggccatt ctgcagagca gaaggtaacc
      361 caacgtctct tcttgacatc taccgactgg ttgtgagcga tccgctcgac atctttccag
      421 tgacctaagg tcaaacttaa gggagtggta acagtctggc cctaattttc agacaaatac
      481 agaaacacag tcagacagag acaacacaga acgatgctgc agcgctgcag cagacaagac
      541 gcgcggcttc ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg
      601 ttctcgtctc ctaccagaac cacatatcct gacggggtcg gattccacat cgactccctt
      661 cctcaggtcg ggccacaaaa acggccccca aagtccctgg gacgtctccc agggttgcgg
      721 ccgggtgttc agaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct
      781 aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac
      841 agataagttg ctggccagct tgcctcccgg tggtgggtcg gtggtccctg ggcaggggtc
      901 tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca
      961 gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     1021 ttggatac
//
