LOCUS       sequence-80604-e        8920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PhyB-mCFP with prenylation sequence from Kras, for 
            red-light-induced recruitment of PIF to the plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL204
FEATURES             Location/Qualifiers
     source          1..8920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3667..3669,3670..3672,3673..4383)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3712..3733)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3973..3992)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4320..4341
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4472..4490)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4473..4490)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4510..4527)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4516..4740
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4786..5214
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4873..4892)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5083..5104
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5223..5243)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5228..5558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5409..5544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5471..5490
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5625..6419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5679..5698)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6289..6308
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6593..6714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6630..6649)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6684..6703
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6763..6779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6763..6779)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6776..6798)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6787..6803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6811..6817,6818..6835,6836..6841))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6856..6877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6994..7011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7165..7753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7245..7264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7924..8715,8716..8784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8547..8566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8785..8889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atggtttccg gagtcggggg tagtggcggt ggccgtggcg gtggccgtgg cggagaagaa
      961 gaaccgtcgt caagtcacac tcctaataac cgaagaggag gagaacaagc tcaatcgtcg
     1021 ggaacgaaat ctctcagacc aagaagcaac actgaatcaa tgagcaaagc aattcaacag
     1081 tacaccgtcg acgcaagact ccacgccgtt ttcgaacaat ccggcgaatc agggaaatca
     1141 ttcgactact cacaatcact caaaacgacg acgtacggtt cctctgtacc tgagcaacag
     1201 atcacagctt atctctctcg aatccagcga ggtggttaca ttcagccttt cggatgtatg
     1261 atcgccgtcg atgaatccag tttccggatc atcggttaca gtgaaaacgc cagagaaatg
     1321 ttagggatta tgcctcaatc tgttcctact cttgagaaac ctgagattct agctatggga
     1381 actgatgtga gatctttgtt cacttcttcg agctcgattc tactcgagcg tgctttcgtt
     1441 gctcgagaga ttaccttgtt aaatccggtt tggatccatt ccaagaatac tggtaaaccg
     1501 ttttacgcca ttcttcatag gattgatgtt ggtgttgtta ttgatttaga gccagctaga
     1561 actgaagatc ctgcgctttc tattgctggt gctgttcaat cgcagaaact cgcggttcgt
     1621 gcgatttctc agttacaggc tcttcctggt ggagatatta agcttttgtg tgacactgtc
     1681 gtggaaagtg tgagggactt gactggttat gatcgtgtta tggtttataa gtttcatgaa
     1741 gatgagcatg gagaagttgt agctgagagt aaacgagatg atttagagcc ttatattgga
     1801 ctgcattatc ctgctactga tattcctcaa gcgtcaaggt tcttgtttaa gcagaaccgt
     1861 gtccgaatga tagtagattg caatgccaca cctgttcttg tggtccagga cgataggcta
     1921 actcagtcta tgtgcttggt tggttctact cttagggctc ctcatggttg tcactctcag
     1981 tatatggcta acatgggatc tattgcgtct ttagcaatgg cggttataat caatggaaat
     2041 gaagatgatg ggagcaatgt agctagtgga agaagctcga tgaggctttg gggtttggtt
     2101 gtttgccatc acacttcttc tcgctgcata ccgtttccgc taaggtatgc ttgtgagttt
     2161 ttgatgcagg ctttcggttt acagttaaac atggaattgc agttagcttt gcaaatgtca
     2221 gagaaacgcg ttttgagaac gcagacactg ttatgtgata tgcttctgcg tgactcgcct
     2281 gctggaattg ttacacagag tcccagtatc atggacttag tgaaatgtga cggtgcagca
     2341 tttctttacc acgggaagta ttacccgttg ggtgttgctc ctagtgaagt tcagataaaa
     2401 gatgttgtgg agtggttgct tgcgaatcat gcggattcaa ccggattaag cactgatagt
     2461 ttaggcgatg cggggtatcc cggtgcagct gcgttagggg atgctgtgtg cggtatggca
     2521 gttgcatata tcacaaaaag agactttctt ttttggtttc gatctcacac tgcgaaagaa
     2581 atcaaatggg gaggcgctaa gcatcatccg gaggataaag atgatgggca acgaatgcat
     2641 cctcgttcgt cctttcaggc ttttcttgaa gttgttaaga gccggagtca gccatgggaa
     2701 actgcggaaa tggatgcgat tcactcgctc cagcttattc tgagagactc ttttaaagaa
     2761 tctgaggcgg ctatgaactc taaagttgtg gatggtgtgg ttcagccatg tagggatatg
     2821 gcgggggaac aggggattga tgagttaggt gcagttgcaa gagagatggt taggctcatt
     2881 gagactgcaa ctgttcctat attcgctgtg gatgccggag gctgcatcaa tggatggaac
     2941 gctaagattg cagagttgac aggtctctca gttgaagaag ctatggggaa gtctctggtt
     3001 tctgatttaa tatacaaaga gaatgaagca actgtcaata agcttctttc tcgtgctttg
     3061 agaggggacg aggaaaagaa tgtggaggtt aagctgaaaa ctttcagccc cgaactacaa
     3121 gggaaagcag tttttgtggt tgtgaatgct tgttccagca aggactactt gaacaacatt
     3181 gtcggcgttt gttttgttgg acaagacgtt actagtcaga aaatcgtaat ggataagttc
     3241 atcaacatac aaggagatta caaggctatt gtacatagcc caaaccctct aatcccgcca
     3301 atttttgctg ctgacgagaa cacgtgctgc ctggaatgga acatggcgat ggaaaagctt
     3361 acgggttggt ctcgcagtga agtgattggg aaaatgattg tcggggaagt gtttgggagc
     3421 tgttgcatgc taaagggtcc tgatgcttta accaagttca tgattgtatt gcataatgcg
     3481 attggtggcc aagatacgga taagttccct ttcccattct ttgaccgcaa tgggaagttt
     3541 gttcaggctc tattgactgc aaacaagcgg gttagcctcg agggaaaggt tattggggct
     3601 ttctgtttct tgcaaatccc gagcgaattc gatagtgctg gtagtgctgg tagtgctggt
     3661 actagtatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3721 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3781 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3841 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac
     3901 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3961 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4021 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4081 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag
     4141 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4201 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4261 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4321 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4381 aaggctagcg gtaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat tatgtaagcg
     4441 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
     4501 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4561 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4621 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4681 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4741 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4801 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4861 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4921 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
     4981 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5041 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5101 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
     5161 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     5221 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     5281 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5341 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5401 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5461 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5521 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5581 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5641 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5701 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5761 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5821 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5881 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5941 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6001 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6061 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6121 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6181 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6241 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6301 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6361 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6421 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6481 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6541 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6601 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6661 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6721 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6781 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6841 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6961 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7021 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7081 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7141 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     7381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7561 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7741 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7801 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7861 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7921 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7981 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8041 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8101 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8161 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8221 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8281 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8341 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8401 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8461 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8521 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8581 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8641 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8701 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8761 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8821 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8881 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
