LOCUS       sequence-80634-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Heterologous expression of E. coli CoA biosynthetic enzyme, PanK 
            (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for 
            nickel affinity purification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Brand LA, Strauss E
  TITLE     Characterization of a new pantothenate kinase isoform from 
            Helicobacter pylori.
  JOURNAL   J Biol Chem. 2005 May 27;280(21):20185-8. Epub 2005 Mar 28.
  PUBMED    15795230
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-05-27"; volume: "280(21)"; pages: "20185-8. Epub
            2005 Mar 28"
COMMENT     Sequence Label: pET28a-Ec.coaA
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgagtata aaagagcaaa cgttaatgac gccttaccta
      121 cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac gttatcggaa
      181 gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga agttgccgag
      241 atctatttac ctttgtcacg tttgctgaac ttctatataa gctcgaatct gcgccgtcag
      301 gcagttctgg aacagtttct tggtactaac gggcaacgca ttccttacat tatcagtatt
      361 gctggcagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc gctattaagc
      421 cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct tcaccctaat
      481 caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc gtatgatatg
      541 catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt tacagcccct
      601 gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt tgttcagcct
      661 gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta tccacacgat
      721 ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga tgcaccggaa
      781 gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg ggcttttacc
      841 gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc gattaagact
      901 gccatgacat tgtggaaaga gatcaactgg ctgaacttaa
//
