LOCUS       sequence-80635-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT 
            (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for 
            nickel affinity purification. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Strauss E, Begley TP
  TITLE     The antibiotic activity of N-pentylpantothenamide results from its 
            conversion to ethyldethia-coenzyme a, a coenzyme a antimetabolite.
  JOURNAL   J Biol Chem. 2002 Dec 13;277(50):48205-9. Epub 2002 Oct 7.
  PUBMED    12372838
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-12-13"; volume: "277(50)"; pages: "48205-9. Epub
            2002 Oct 7"
COMMENT     Sequence Label: pET28a-Ec.coaD (pESC106)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             612..629
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(682..700)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      696..743
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgcaaaaa cgggcgattt atccgggtac tttcgatccc
      121 attaccaatg gtcatatcga tatcgtgacg cgcgccacgc agatgttcga tcacgttatt
      181 ctggcgattg ccgccagccc cagtaaaaaa ccgatgttta ccctggaaga gcgtgtggca
      241 ctggcacagc aggcaaccgc gcatctgggg aacgtggaag tggtcgggtt tagtgattta
      301 atggcgaact tcgcccgtaa tcaacacgct acggtgctga ttcgtggcct gcgtgcggtg
      361 gcagattttg aatatgaaat gcagctggcg cacatgaatc gccacttaat gccggaactg
      421 gaaagtgtgt ttctgatgcc gtcgaaagag tggtcgttta tctcttcatc gttggtgaaa
      481 gaggtggcgc gccatcaggg cgatgtcacc catttcctgc cggagaatgt ccatcaggcg
      541 ctgatggcga agttagcgta gcgttggatc cgaattcgag ctccgtcgac aagcttgcgg
      601 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      661 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      721 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      781 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      841 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttccc
//
