LOCUS       sequence-80636-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK 
            (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity
            purification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Strauss E, Begley TP
  TITLE     The antibiotic activity of N-pentylpantothenamide results from its 
            conversion to ethyldethia-coenzyme a, a coenzyme a antimetabolite.
  JOURNAL   J Biol Chem. 2002 Dec 13;277(50):48205-9. Epub 2002 Oct 7.
  PUBMED    12372838
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-12-13"; volume: "277(50)"; pages: "48205-9. Epub
            2002 Oct 7"
COMMENT     Sequence Label: pET28a-Ec.coaE (pESC124)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             752..769
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(822..840)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      836..883
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgaggtat atagttgcct taacgggagg cattggcagt
      121 ggcaagagta ccgttgccaa tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat
      181 attattgcgc gtcaggtggt tgaaccaggt gcacctgcgc tacatgccat tgctgatcac
      241 tttggcgcta acatgattgc tgctgatgga acattgcagc gccgggcctt gcgcgagcgg
      301 atcttcgcca acccggaaga gaaaaactgg cttaacgccc tgctgcatcc gctgattcag
      361 caagagacgc aacaccagat ccagcaagct acttccccct atgtactgtg ggttgtgcca
      421 ttgctggtag aaaactcact gtataaaaaa gcgaatcgag tgcttgtggt ggatgtcagc
      481 ccagaaacgc aacttaagcg caccatgcag cgcgatgatg taactcgcga gcatgtcgaa
      541 caaatccttg ctgctcaggc aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt
      601 gataataacg gcgcaccgga tgctatcgca tcggatgttg cccgcctgca cgcacactat
      661 ttgcagcttg cgtcgcagtt tgtctcacag gaaaaaccgt aatgcggatc cgaattcgag
      721 ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      781 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      841 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      901 atccggattg gcgaatgg
//
