LOCUS       sequence-80638-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Heterologous expression of E. coli CoA biosynthetic enzyme, 
            PPCS_PPCDC - phosphopantothenoylcysteine 
            synthetase_phosphopantothenoylcysteine decarboxylase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Strauss E, Kinsland C, Ge Y, McLafferty FW, Begley TP
  TITLE     Phosphopantothenoylcysteine synthetase from Escherichia coli. 
            Identification and characterization of the last unidentified 
            coenzyme A biosynthetic enzyme in bacteria.
  JOURNAL   J Biol Chem. 2001 Apr 27;276(17):13513-6. Epub 2001 Mar 13.
  PUBMED    11278255
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-04-27"; volume: "276(17)"; pages: "13513-6. Epub
            2001 Mar 13"
COMMENT     Sequence Label: pET28a-Ec.coaBC (pCLK1210)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgagcctg gccggtaaaa aaatcgttct
      121 cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc gtttgcgcga
      181 tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta tcaccccact
      241 tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc cggcagccga
      301 agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc tcgcccctgc
      361 cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg tatcgacgat
      421 ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc agatgtaccg
      481 tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc tcatctgggg
      541 gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc tcgatccgtt
      601 aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga aacatctgaa
      661 cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt atatctctaa
      721 tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc gtggcgcgaa
      781 cgtcacgctg gtatcaggtc cggtttcact accgacgcca ccgtttgtta aacgtgttga
      841 tgtgatgacc gcgctggaaa tgg
//
