LOCUS       sequence-80653-e       11735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of humanized Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ222
FEATURES             Location/Qualifiers
     source          1..11735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..4122
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             19..4122
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             19..4122
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4135..4155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4173..4420
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4189..4207)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      4790..5571
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(5654..5670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5654..5670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5667..5689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5678..5694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5702..5708,5709..5726,5727..5732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5747..5768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5885..5902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6056..6644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6136..6155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6815..7606,7607..7675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7438..7457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7676..7780)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7848..7866
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7904..7926)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8026..8045
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8062..8517)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8199..8220)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8411..8430
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8715..8737
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8729..8746
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8730..8746
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8997..9404
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             9405..10499
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        10992..11733
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
ORIGIN
        1 tgcaggtcga ctctagagat ggacaagaag tactccattg ggctcgatat cggcacaaac
       61 agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt
      121 ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac
      181 tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc
      241 agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat
      301 gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag
      361 cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc
      421 atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc
      481 tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg
      541 aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag
      601 cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct
      661 aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag
      721 aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct
      781 aacttcgacc tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat
      841 ctcgacaatc tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag
      901 aacctgtcag acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa
      961 gctccgctga gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg
     1021 ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag
     1081 tctaaaaatg gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa
     1141 tttattaagc ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagcttaac
     1201 agagaagatc tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt
     1261 cacctgggcg aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa
     1321 gataacaggg aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc
     1381 ctcgcccggg gaaattccag attcgcgtgg atgactcgca aatcagaaga gaccatcact
     1441 ccctggaact tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg
     1501 atgactaact ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg
     1561 tacgagtact tcacagttta taacgagctc accaaggtca aatacgtcac agaagggatg
     1621 agaaagccag cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag
     1681 acgaaccgga aagttaccgt gaaacagctc aaagaagact atttcaaaaa gattgaatgt
     1741 ttcgactctg ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat
     1801 cacgatctcc tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac
     1861 attcttgagg acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa
     1921 cgcttgaaaa cttacgctca tctcttcgac gacaaagtca tgaaacagct caagaggcgc
     1981 cgatatacag gatgggggcg gctgtcaaga aaactgatca atgggatccg agataagcag
     2041 tctggaaaga caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg
     2101 cagttgatcc atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct
     2161 ggccaggggg acagtcttca cgagcacatc gctaatcttg caggtagccc agctatcaaa
     2221 aagggaatac tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat
     2281 aagcccgaga atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag
     2341 aagaacagta gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa
     2401 atccttaagg aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac
     2461 tacctgcaga acggcaggga catgtacgtg gatcaggaac tggacatcaa tcggctctcc
     2521 gactacgacg tggatcatat cgtgccccag tcttttctca aagatgattc tattgataat
     2581 aaagtgttga caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa
     2641 gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa
     2701 cggaagttcg ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc
     2761 ggcttcatca aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt
     2821 ctcgattcac gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa
     2881 gttattactc tgaagtctaa gctggtctca gatttcagaa aggactttca gttttataag
     2941 gtgagagaga tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc
     3001 actgcactta tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg agactataaa
     3061 gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct
     3121 aagtacttct tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat
     3181 ggagagattc ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg
     3241 gacaagggta gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc
     3301 gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg
     3361 aacagcgaca agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc
     3421 gattctccta cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct
     3481 aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc
     3541 gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata aagaggtcaa aaaagacctc
     3601 atcattaagc ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc
     3661 gctagtgcgg gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat
     3721 ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag
     3781 aagcagctgt tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc
     3841 gaattctcca aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac
     3901 aataagcaca gggataagcc catcagggag caggcagaaa acattatcca cttgtttact
     3961 ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag
     4021 cggtacacct ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg
     4081 ctctatgaaa caagaatcga cctctctcag ctcggtggag acagcagggc tgaccccaag
     4141 aagaagagga aggtgtgatc tcgttgctcg agtcatgtaa ttagttatgt cacgcttaca
     4201 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     4261 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     4321 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     4381 gcttgagaag gttttgggac gctcgaaggc tttaatttgc gatccccggg taccgagctc
     4441 gaattccaac cttccaattc attaaatcga gtcctccatc agatgattta caaggttttg
     4501 catagaatcc ctaagtaaga gaagttgtta gtgatttctt caagtttact aaaatttaca
     4561 tacaaatgga tgatctcttc tccattgttt cctttcttct tgcaaacgcg ttttacaaag
     4621 agatgacatg ctattttctg attatttttt tctatagttt tctattttgt acgtccttgg
     4681 gagtccgaaa tgtaaaatcg gggtatggtt agtagcggca gcgttcatta tggtatataa
     4741 aagataaaaa gtaggggcaa gcgagacaga atgggataca agggcatcgt ctataattat
     4801 agctaaaaat tgtattttaa tttgtatttt ttgtaatttt atttctcatt gttttactta
     4861 aaatgaatgc gaattagaga aaacttcaac gaaatgtcaa aataagctca gcaaaatata
     4921 caattttagg gaaagcgatc agcaacttat tctccgttgt agattctttt tcttcataaa
     4981 ttccaacaat gtaactgcca attgattttt ctctagatgc taataaattg aaactaatga
     5041 atataataac acaggttttg ataaattgta aactcttact caaaaaaaga atgttaaaac
     5101 taatttaacc tttttataaa ggctcatggc gttaaagagg atagtaaaaa ttctacaatt
     5161 ttttaattta atgtcacaat tgtcttgcga atataattct attaattttc gctaactgct
     5221 gttttagtac tattactttc aatatgcctt atatgcaact ttaattcatg aagaatgcaa
     5281 tatttttgca atctttcttg tactgagcgc tttttatcat ttagtactca ttatttaatt
     5341 tttcagtaaa gggcaataaa taaatttgta gaagatgcaa tgtaatctcc tctatccttt
     5401 tgctcatatg tttatgagta tacctagtct agaaaggctt gtatttaaaa tattattcaa
     5461 taaaaattca caatttttac gacatgtgct accattcact taacttcctg attataaaat
     5521 tggttcgttt atactaatta cttaagtacc tttaactaaa caaaatgcct atatatatat
     5581 taatttacaa tgagtgtcag ataagtcact atgtccgagt ggttaaggag ttagactcga
     5641 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5701 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5761 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5821 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6481 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7801 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     7861 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7921 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7981 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     8041 ttgtactgag agtgcaccat aaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa
     8101 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     8161 aatcaaaaga atagcccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     8221 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     8281 cactacgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     8341 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     8401 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     8461 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtact
     8521 atggttgctt tgacgtatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8581 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8641 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     8701 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgtcga
     8761 ggagaacttc tagtatatct acatacctaa tattattgcc ttattaaaaa tggaatccca
     8821 acaattacat caaaatccac attctcttca aaatcaattg tcctgtactt ccttgttcat
     8881 gtgtgttcaa aaacgttata tttataggat aattatactc tatttctcaa caagtaattg
     8941 gttgtttggc cgagcggtct aaggcgcctg attcaagaaa tatcttgacc gcagttaact
     9001 gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt
     9061 tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
     9121 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
     9181 tgggagaaaa aggaaaggtg agagccgcgg aaccggcttt tcatatagaa tagagaagcg
     9241 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat
     9301 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca
     9361 tttcagcaat atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga
     9421 agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg
     9481 ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa
     9541 ttggtggtgc tgctatcgat gctacaggtg tcccacttcc agatgaggcg ctggaagcct
     9601 ccaagaaggt tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta
     9661 gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact
     9721 taagaccatg taactttgca tccgactctc ttttagactt atctccaatc aagccacaat
     9781 ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta
     9841 agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag
     9901 aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc
     9961 ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg
    10021 tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa ttgattgatt
    10081 ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca
    10141 acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt
    10201 tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac
    10261 catgccacgg ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct
    10321 tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg
    10381 aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca
    10441 acagtaccac ggaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa
    10501 aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa
    10561 acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc
    10621 tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt
    10681 gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa
    10741 ggaggagggc accacacaaa aagttaggtg taacagaaaa tcatgaaact atgattccta
    10801 atttatatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa aaaacactca
    10861 atgacctgac catttgatgg agtttaagtc aataccttct tgaaccattt cccataatgg
    10921 tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttaacgacgt agtcgatcga
    10981 caagcttgca tgccctacaa caactaagaa aatggctatc atgcggaagc tggtgagaag
    11041 aacagcatcg ggacaaggga aggaagaaca aagacaaaga aaacaaaaga aagcaattga
    11101 aaacaaaaca aaacaatttt cattccttct cttatcattc cttttctttt cttttctctc
    11161 attcaacgca ctccatcgta tccgtattcc tcttattttt tctctttctc tatatccatt
    11221 tctttctctc taggtgtgtc ctctctctct cttcaatttc tctactccgc attccaacgc
    11281 atccttcccc caacctccca tttcctcctt acggcccgat agcgatcgtc tttccctcgc
    11341 tatcactcgc taccggcccc tcctctgcac cgtaacctcc tacgtattta ccatatcata
    11401 aagttttttc cgacgcttat cgctgacccc ctgtcgccct cctattggct tccggattat
    11461 cttcttgtcc ataaggtgat ccatgcttcc tgaagattcc cgaaatgtgt ccactttggc
    11521 ggggaatcat tccatccact tctttctctc tcgctttcct cattcggcgc tccccttccg
    11581 cgtctcattg gtcttccgct ccgtttttgc tttgccgatg ttacttgggg agaggtgcga
    11641 taatcctttc gcaaaaactc ggtttgacgc ctcccatggt ataaatagtg ggtggtggac
    11701 aggtgccttc gcttttcttt aagcaagaga attgc
//
