LOCUS       sequence-80657-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Saccharomyces cerevisiae expression vector with GAL1 
            promoter and C-terminal 3C protease cleavage site, GFP and 10x His 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pYEXC3GH
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(33..74)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(96..125)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(132..842)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(849..872)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             899..919
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             923..943
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actcaatggt gatggtgatg atgaccggta cgcgtagaat cgagaccgag gagagggtta
       61 gggataggct taccttcgaa gggccctcta gattaatgat ggtgatgatg atggtgatga
      121 tgatgactag ttttgtacaa ttcatccata ccatgggtaa taccagcagc agtaacaaat
      181 tctaacaaga ccatgtggtc tctcttttcg tttggatctt tggataaggc agattgagtg
      241 gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt attttgttga
      301 taatggtcag ctaattgaac agaaccatct tcaatgttgt gtctaatttt gaagttaact
      361 ttgataccat tcttttgttt gtcagccatg atgtaaacat tgtgagagtt atagttgtat
      421 tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt taattcgatt
      481 ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta gttaccgtca
      541 tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga cttgaaaaag
      601 tcatgttgtt tcatatgatc tgggtatctc gcaaaacatt gaacaccata accgaaagta
      661 gtgactaagg ttggccatgg aactggcaat ttaccagtag tacaaataaa ttttaaggtc
      721 aatttaccgt aagtagcatc accttcacct tcaccggaga cagaaaattt gtgaccatta
      781 acatcaccat ctaattcaac caaaattggg acaacaccag tgaataattc ttctccttta
      841 gagaattgtg gaccttgaaa caaaacttct aatgcagaag agctgaacta gtggatcccc
      901 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagctagcg aat
//
