LOCUS       sequence-80659-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expresses FLAG/HA-tagged MOV10 K530A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa
            S, Dieterich C, Landthaler M
  TITLE     MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target 
            degradation by translocation along 3' UTRs.
  JOURNAL   Mol Cell. 2014 May 22;54(4):573-85. doi: 
            10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10.
  PUBMED    24726324
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.017"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "573-85"
COMMENT     Sequence Label: pFRT/FLAG/HA MOV10 K530A
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             144..167
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             168..194
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    210..234
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg agctcggatc gatccatcga tagtgtggtg
      121 gaattctgat cgccgccgcc atggactaca aggacgacga tgacaagtac ccttatgacg
      181 tgcccgatta cgctttgaac cagatatcaa caagtttgta caaaaaagca ggctccacca
      241 tgggaaccaa ttcagtcgac atgcccagta agttcagctg ccggcagctc cgggaggcgg
      301 gccagtgttt cgagagtttc ctggtcgttc ggggactgga catggagaca gatcgcgagc
      361 ggctgcggac catttataac cgcgacttca agatcagctt tgggaccccc gcccctggct
      421 tctcctccat gctgtatgga atgaagattg caaatctggc ctacgtcacc aagactcggg
      481 tcaggttctt cagactcgac cgctgggccg acgtgcggtt cccagaaaag aggagaatga
      541 agctggggtc agatatcagc aaacaccaca agtcactgct agccaagatc ttttatgaca
      601 gggctgagta tcttcatggg aaacatggtg tggatgtgga agtccagggg ccccatgaag
      661 cccgagatgg gcagctcctt atccgcctgg atttgaaccg caaagaggtg ctgaccctga
      721 ggcttcggaa tggcggaacc cagtctgtta ccctcactca cctcttccca ctctgccgga
      781 caccccagtt tgctttctac aatgaagacc aggagttgcc ctgtccactg ggccccggtg
      841 aatgctatga actccatgtc cattgtaaga ccagctttgt gggctacttc ccagccacag
      901 tgctctggga gctgctggga cctggggagt cgggttcaga aggagccggc acattctaca
      961 ttgcccgctt cttggctgcc gtcgccc
//
