LOCUS       sequence-80661-e         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  p2attNG landing pad plasmid with 5 and 3kb homology arms to the 
            human H11 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attNG-H11-long
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(14..35)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(275..294)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     622..643
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
       61 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      121 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      181 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      241 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      301 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      361 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      421 ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa
      481 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      541 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      601 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtga
//
