LOCUS       sequence-80668-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL5 and attL2 sites flanking a CmR and 
            ccdB gene. In our hands, the multisite reaction still proceeds 
            efficiently. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB80
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(137..164)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(256..342)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     391..413
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     405..422
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     406..422
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    437..532
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(565..570,571..588,589..595)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(715..734)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgcg agccaggaag
      121 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
      181 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
      241 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      301 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
      361 ctctcgcgtt aacgcttgca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
      421 gtcttaagct cgggcccaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
      481 caaattgatg agcaatgctt ttttataatg ccaactttgt atacaaaagt tgccgagctc
      541 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
      601 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      661 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      721 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      781 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      841 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
      901 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
      961 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1021 g
//
