LOCUS       sequence-80673-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL1 and attR5 sites. Between the att 
            sites are two FokI coding sequences transcriptionally linked by a 
            T2A sequence. There is a nos-T after the last FokI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB91
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     157..173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     157..173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     178..197
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        178..196
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(214..337)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(382..634)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggtagcag cgaagtcgag
       61 gcatttctgt cctggctggt ccagaggagg catgatgata tatttttatc ttgtgcaatg
      121 taacatcaga gattttgaga cacgggccag agctgccagg aaacagctat gaccatgtaa
      181 tacgactcac tataggggat atcagctgga tggcatagtg actggatatg ttgtgtttta
      241 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      301 ttttacgttt ctcgttcaac ttttgtatac aaagttgcga attcgccctt tccggaagct
      361 gtcgaggggg gatcaattcc cgatctagta acatagatga caccgcgcgc gataatttat
      421 cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
      481 taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct
      541 taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc
      601 ttaagaaact ttattgccaa atgtttgaac gatcggacgt cctattaaaa gtttatctcg
      661 ccgttattaa atttccgtct cacttcctct aaggttaatg tgccggcttt aatcatttct
      721 ccaccaatta aaagctcttc tacactaaga acagctccat tacaattagt gatatgattt
      781 aatcgtgtaa gctgagcttt gtagtttcct ttaaagtgac cactcacaaa taaaaactta
      841 aattccgtta cagaagatgg atagactttc caccattcat tagggttgag atgtttgttt
      901 cgtgtttgat tttcttcgac atatcgctcc atttcatctg cttggccaat tggcagatta
      961 taacctccgc tataagcttt agtatccacg atcacaccgt aatcaatagg agatccgaca
     1021 gt
//
