LOCUS       sequence-80691-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 
            849,865,877,907,980,1006 mutated to Ala .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggaatgttt ccagactgaa ccctattcta ccctccaaag cccctgcggt atctcctctc
       61 ataggaaatg gcacacagtc caataacaac tatagctcgg gtgggcccgg gaccctcctc
      121 ccgagcagaa gtgacctgtc aggtgtagac ttcacagtgc tgaacacact caacaggaga
      181 gacgcaaata ccagcactat cagctctgcc tacctgagca gccgcagatc cgcaggcatc
      241 tccccctgct tttccagccg caggtccgca gaggcgtccc aggctgaagg gcgaccccag
      301 aatgtgagtg tggctgactc ctacgatccc atctccacag atgcttcaag gaggtccgca
      361 gaggccagcc agggtgacgg gctgcccagt ctgctcagcc tcacacccgt gcagcagtac
      421 cgccttaagg ccaagtatgc agctgccaca ggtggcccac cacccacacc tctgccccac
      481 atggagaggc tgtcactgaa gaccaagatg gccttgctag gggaagggag ggactctggg
      541 gtgaccctgc ctccagtcca tcctcctcga agatgcgcag atgggggagg tcacacatac
      601 agagggcgtc acctgatgcc tcatgatgca ctagcgaaca gtgtgaggag agccgcagac
      661 cctgtgagga cagtctcgga gaacatgtca cttgccaggg ttcaacgctt cagcagcctc
      721 aatagcttta atcctccaaa tttgcctcca tcggtggaaa agcgttcact ggtcttacag
      781 aactacaccc ggcaggagag cagccaaccc cggtacttcc aggcatcccc ttgccctccc
      841 agcatcacag agaatgttgc cctggaggct ctgaccatgg atgctgatgc taacttga
//
