LOCUS       sequence-80692-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of FtsZ-mEos2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Buss J, Coltharp C, Huang T, Pohlmeyer C, Wang SC, Hatem C, Xiao J
  TITLE     In vivo organization of the FtsZ-ring by ZapA and ZapB revealed by 
            quantitative super-resolution microscopy.
  JOURNAL   Mol Microbiol. 2013 Sep;89(6):1099-120. doi: 10.1111/mmi.12331. Epub
            2013 Aug 14.
  PUBMED    23859153
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/mmi.12331";
            journalName: "Mol Microbiol"; date: "2013-09"; volume: "89"; issue: 
            "6"; pages: "1099-120"
COMMENT     Sequence Label: pJB042 (FtsZ-mEos2)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(26..43)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(26..42)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(35..57)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     200..218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(242..256,257..262,263..279,280..286)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    262..278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    294..310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             329..340
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
       61 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      121 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcctaagaaa ccattattat
      181 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagaa
      241 atcataaaaa atttatttgc tttgtgagcg gataacaatt ataatagatt caattgtgag
      301 cggataacaa tttcacacag aattcattaa agaggagaaa ttaactacta gtatgtttga
      361 accaatggaa cttaccaatg acgcggtgat taaagtcatc ggcgtcggcg gcggcggcgg
      421 taatgctgtt gaacacatgg tgcgcgagcg cattgaaggt gttgaattct tcgcggtaaa
      481 taccgatgca caagcgctgc gtaaaacagc ggttggacag acgattcaaa tcggtagcgg
      541 tatcaccaaa ggactgggcg ctggcgctaa tccagaagtt ggccgcaatg cggctgatga
      601 ggatcgcgat gcattgcgtg cggcgctgga aggtgcagac atggtcttta ttgctgcggg
      661 tatgggtggt ggtaccggta caggtgcagc accagtcgtc gctgaagtgg caaaagattt
      721 gggtatcctg accgttgctg tcgtcactaa gcctttcaac tttgaaggca agaagcgtat
      781 ggcattcgcg gagcagggga tcactgaact gtccaagcat gtggactctc tgatcactat
      841 cccgaacgac aaactgctga aagttctggg ccgcggtatc
//
