LOCUS       sequence-80696-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH87644
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(157..261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     329..347
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    387..442
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
       61 caaaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
      121 ttcggtcaag gttctggacc agttgcgtga gcgcatactc ttcctttttc aatattattg
      181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
      301 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtccact
      361 gaagagccac ttcgtggtct cacattgtaa ttgtaaatag taattgtaat gttgtttgtt
      421 gtttgttgtt gttggtaatt gttgtaaaaa tacagtattc aaagcggaga ggaaaatata
      481 tgaatttata taggcgggtt tatctcttac aactttattt tcggcctttc aaaaaaataa
      541 ttaaaatcga cagacacgaa tcatttcgac cacaggtaaa gataacgtga cctggctgtc
      601 agacagcctt ttccctcgtg ttaactaatt tttaaactaa ttaatcatct cagcccttgg
      661 attagttctt ttgctttgat ggcttcatga ctgtgacctg ctcgatccgc gtgttacatg
      721 acagctccgt ttttttagtg gttaacttaa accgagtcaa tccaggcaac gttagtcgtc
      781 gtcgtggttg gcttgttcaa ttagatttca tacaattcaa cgta
//
