LOCUS       sequence-80706-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  p2attNG landing pad plasmid with 5 and 3kb homology arms to the 
            human H11 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attNG-H11-long
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    73..297
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     286..303
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     intron          371..436
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ctcactaaag ggaacaaaag ctgggtaccg ggccccccct cgagccccag ctggttcttt
       61 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
      121 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
      181 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
      241 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
      301 aggctgatca gcgagctcta ggatctgcat tccaccactg ctcccattca tcagttccat
      361 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
      421 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
      481 caccacagaa gtaaggttcc ttcacaaaga gatcgcctga cacgatttcc tgcacaggct
      541 tgagccatat actcatacat cgcatcttgg ccacgttttc cacgggtttc aaaattaatc
      601 tcaagttcta cgcttaacgc tttcgcctgt tcccagttat taatatattc aacgctagaa
      661 ctcccctcag cgaagggaag gctgagcact acacgcgaag caccatcacc gaaccttttg
      721 ataaactctt ccgttccgac ttgctccatc aacggttcag tgagacttaa acctaactct
      781 ttcttaatag tttcggcatt atccactttt
//
