LOCUS       sequence-80709-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by 
            rice snoRNA U3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGE31
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             109..174
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             181..201
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta caaaaaagca
       61 ggcttcgaag gagatagaac caattctcta aggaaatact taaccatgga ctataaggac
      121 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
      181 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
      241 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
      301 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
      361 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
      421 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      481 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      541 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
      601 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
      661 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
      721 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
      781 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
      841 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
      901 gcccagctgc ccggcgagaa gaagaatggc ctggttcgga aacctgattg cccttgagcc
      961 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aactgcagct
     1021 gagcaaggac acctacgacg acgacctgga caacctg
//
