LOCUS       sequence-80711-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of synthetic 
            trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC32B-AtTAS1c-B/c
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             372..677
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     556..575
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa acgcgtggag ccggcttact aaaagccaga
       61 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
      121 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca
      181 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac
      241 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga
      301 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag
      361 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      421 ttgtggatgt acagagtgat attattgaca cgcccggccg acggatggtg atccccctgg
      481 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
      541 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt tccgttatcg
      601 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
      661 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca cctcgacggt
      721 ctcagaacta gaaaagacat tggacatatt ccaggatatg caaaagaaaa caatgaatat
      781 tgttttgaat gtgttcaagt aaatgagatt ttcaagtcgt ctaaagaaca gttgctaata
      841 cagttactta tttcaataaa taattggttc taataataca aaacatattc gaggatatgc
      901 agaaaaaa
//
