LOCUS       sequence-80713-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible entry vector for direct cloning of synthetic 
            trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pENTR-AtTAS1c-B/c
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(579..678)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(694..711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(694..710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(703..725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggctccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tggtctctta attttctaag atccaccgat aaatggtcta ttcgcttgta
      181 gagaaaaatc acaacaaaac aatgctgttt catctcgaaa acatccgtaa cccaacgaaa
      241 tcaaagaaga ggacgggaag gaacgaaact gacacatggc tggtgaaaat tggaccggga
      301 cattgaacct aacttctgac aaacgcaatc tcaaactaac tatagacgac gctctactcg
      361 atactcaaaa gaagtaaacg aaagtacctt tctatgagac aagaccatga ctcgatctaa
      421 cggcggaaca tgatctaaga acccatctca cttttgtgtt aagatggact tacagatcgt
      481 atttgcagag cttgacggcg ctcttgtttt tctctttttg gtatttgacg tcgggcttag
      541 ccgtttaggt ttaggtttgg tgaagggggc ggccgcggag cctgcttttt tgtacaaagt
      601 tggcattata aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca
      661 aaataaaatc attatttggg gcccgagctt aagactggcc gtcgttttac aacgtcgtga
      721 ctgggaaaac atccatgcta gcgttaacgc gagagtaggg aactgccagg catc
//
