LOCUS       sequence-80714-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC123SB-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             302..607
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     486..505
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgtatttgcg cgctgatttt tgcggnataa gaatatatac tgatatgtat acccgaagta
       61 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
      121 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
      181 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
      241 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
      301 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
      361 acagagtgat attattgaca cgcccggccg acggatggtg atccccctgg ccagtgcacg
      421 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
      481 ctggcgcatg atgaccaccg atatggccag tgtgccggtt tccgttatcg gggaagaagt
      541 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
      601 aatataaatg tcaggctccc ttatacacag ccagtctgca cctcgacggt ctcacattgg
      661 ctcttcttac tacaatgaaa aaggccgagg caaaacgcct aaaatcactt gagaatcaat
      721 tctttttact gtccatttaa gctatctttt ataaacgtgt cttattttct atctcttttg
      781 tttaa
//
