LOCUS       sequence-80715-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV IRES Luciferase
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    68..635
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    85..637
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(229..246)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     456..475
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(715..733)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cctactcctt ttcagcccct cactccttct ctaggcgccg gaattagatc tctcgaggtt
       61 aacgaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
      121 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
      181 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
      241 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
      301 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
      361 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
      421 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
      481 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
      541 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
      601 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggaagacgcc
      661 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
      721 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
      781 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
      841 gaagctatga aacgatatgg gctgaaatac aaatcacaga atcgtcgtat gcagtgaaaa
      901 ctctcttcaa
//
