LOCUS       sequence-80716-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC32B-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             366..671
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     550..569
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gatgagtggc agggcggggc gtaaacgcgt ggagccggct tactaaaagc cagataacag
       61 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
      121 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
      181 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
      241 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
      301 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
      361 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
      421 atgtacagag tgatattatt gacacgcccg gccgacggat ggtgatcccc ctggccagtg
      481 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
      541 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtttccgtt atcggggaag
      601 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
      661 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcacctcga cggtctcaca
      721 ttggctcttc ttactacaat gaaaaaggcc gaggcaaaac gcctaaaatc acttgagaat
      781 caattctttt tactgtccat ttaagctatc ttttataaac gtgtcttatt t
//
