LOCUS       sequence-80717-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible entry vector for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pENTR-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             365..670
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     549..568
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atgagtggca gggcggggcg taaacgcgtg gagccggctt actaaaagcc agataacagt
       61 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
      121 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
      181 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
      241 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
      301 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
      361 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
      421 tgtacagagt gatattattg acacgcccgg ccgacggatg gtgatccccc tggccagtgc
      481 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
      541 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtttccgtta tcggggaaga
      601 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
      661 gggaatataa atgtcaggct cccttataca cagccagtct gcacctcgac ggtctcacat
      721 tggctcttct tactacaatg aaaaaggccg aggcaaaacg cctaaaatca cttgagaatc
      781 aattcttttt actgtccatt taagctatct tttataaacg tgtcttattt tctatctctt
      841 ttgtttaaac taagaaacta tagtattttg tctaaaacaa aacat
//
