LOCUS       sequence-80719-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 nickase optimized for nuclear import..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins 
            A, Iyer V, Huang X, Skarnes WC
  TITLE     Efficient genome modification by CRISPR-Cas9 nickase with minimal 
            off-target effects.
  JOURNAL   Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
  PUBMED    24584192
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857."
COMMENT     Sequence Label: pST1374-N-NLS-flag-linker-Cas9-H840A
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctacctgta ctacctccag aacgggcggg acatgtacgt tgaccaggaa ctcgacatca
       61 accggctgtc cgattatgac gtggacgcta ttgttccaca gtccttcctc aaagatgact
      121 ccattgacaa caaggtgctg accagatccg ataagaatcg cggtaagtct gacaatgttc
      181 catcagaaga ggtggtcaag aagatgaaga attactggcg gcagctcctc aacgccaaac
      241 tgatcaccca gcggaagttt gacaatctga ctaaggcaga aagaggaggt ctgagcgaac
      301 tcgacaaggc cggctttatt aagaggcaac tggtcgaaac acgccagatt accaaacacg
      361 tggcacaaat cctcgactct aggatgaaca ctaagtacga tgagaacgat aagctgatca
      421 gggaagtgaa agtgataact ctgaagagca agctggtgtc tgacttccgg aaggactttc
      481 aattctacaa agttcgcgaa ataaacaatt accatcatgc tcacgatgcc tatctcaatg
      541 ctgtcgttgg caccgccctg atcaagaaat accctaaact ggagtctgag ttcgtgtacg
      601 gtgactataa agtctacgat gtgaggaaga tgatagcaaa gtctgagcaa gagattggca
      661 aagccaccgc caagtacttc ttctactcta atatcatgaa tttctttaag actgagataa
      721 ccctggctaa cggcgaaatc cggaagcgcc cactgatcga aacaaacgga gaaacaggag
      781 aaatcgtgtg ggataaaggc agggacttcg caactgtgcg gaaggtgctg tccatgccac
      841 aagtcaatat cgtgaagaag accgaagtgc agaccggcgg attctcaaag gagagcatcc
      901 tgccaaagcg gaactctgac aagctgatcg ccaggaagaa agattgggac ccaaagaagt
      961 atgg
//
