LOCUS       sequence-80721-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC123SB-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..735
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     672..692
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gacctctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
       61 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
      121 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
      181 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
      241 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
      301 attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
      361 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
      421 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
      481 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
      541 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
      601 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
      661 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
      721 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc gagtctacca
      781 tgagcccaga acgacgcccg gccgacatcc gccgtgccac cgaggcggac atgccggcgg
      841 tctgcaccat cgtcaaccac tacatcgaga caagcacggt caacttccgt accgagccgc
      901 aggaaccgca ggagtggacg gacgacctcg
//
