LOCUS       sequence-80727-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC32B-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(151..827)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(194..214)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
       61 cgcggtgagt tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag
      121 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
      181 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
      241 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
      301 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
      361 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
      421 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
      481 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
      541 cttggagtag acgagagtgt cgtgctccac catgttcaca tcaatccact tgctttgaag
      601 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
      661 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
      721 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
      781 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
      841 cttctgaga
//
