LOCUS       sequence-80729-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible entry vector for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pENTR-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(375..380,381..398,399..405)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(525..544)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tccgcggccg cccccttcac ctataggggg gaaaaaaagg
      121 tagtcatcag atatatattt tggtaagaaa atatagaaat gaataatttc acgtttaacg
      181 aagaggagat gacgtgtgtt ccttcgaacc cgagttttgt tcgtctataa atagcacctt
      241 ctcttctcct tcttcctcac ttccatcttt ttagcttcac tatctctcta taatcggttt
      301 tatctttctc taagtcacaa cccaaaaaaa caaagtagag aagaatctgt aagagaccat
      361 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
      421 aggagccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      481 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      541 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      601 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      661 atgctcatcc ggagttccgt atggcaatga aagacggtga gctggtgata tgggatagtg
      721 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      781 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      841 gtg
//
